Journal: Stem Cell Research & Therapy

Article Title: Novel stem cell therapy for cerebral palsy using stem cells from human exfoliated deciduous teeth

doi: 10.1186/s13287-025-04828-y

Figure Lengend Snippet: SHED administration enhances neurogenesis in the hippocampal dentate gyrus and subventricular zone. A Timeline of SHED administration and sample collection for histological evaluation (early time point). B , C Representative images of doublecortin (DCX, green) and bromodeoxyuridine (BrdU, red) double-positive cells in the hippocampal dentate gyrus or striatum. Z-stacks were established to verify that they were truly double-positive. Bar = 10 μm. D – K Representative image of DCX (green) and BrdU (red) immunostaining in the hippocampal dentate gyrus or striatum of each group. The number of DCX/BrdU double-positive cells (white arrow) was increased in the SHED group. Bar = 100 μm. J , K The average number of DCX/BrdU double-positive cells in the hippocampal dentate gyrus or striatum in each group. L Timeline of SHED administration and sample collection for histological evaluation (late time point). M – O Representative image of NeuN (blue) immunostaining in the hippocampal dentate gyrus of each group. Bar = 500 μm P – R Representative image of the higher-magnification view of each group. Bar = 20 μm S – U Representative image of NeuN immunostaining in the cortex of rats of each group. Bar = 500 μm V – X Representative image of a higher-magnification view of each group. Bar = 20 μm. The number of NeuN-positive cells was increased in the SHED group. Y , Z Average Number of NeuN-positive cells in the hippocampal dentate gyrus or cortex of each group. Data are presented as the mean ± SD; Vehicle: n = 8, SHED: n = 8, Sham: n = 8 (early time point), Vehicle: n = 21, SHED: n = 12, Sham: n = 20 (late time point); * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; the Mann–Whitney U test with Holm’s adjustment for multiple comparisons. Values of Cohen’s d are indicated in the graph to represent the effect sizes of group differences

Article Snippet: Adult Rat Hippocampal Neural stem cells (NSC, SCR022, Merck) were plated on plates coated with Poly-L-ornithine (P3655, Merck) and Laminin (CC095, Merck), and cultured in DMEM/F12 (Thermo Fisher Scientific) containing the 2% B-27 supplement (Thermo Fisher Scientific), 1% GlutaMAXTM supplement (Thermo Fisher Scientific), and 20 ng/mL fibroblast growth factor basic protein (GF003, Merck).

Techniques: Immunostaining, MANN-WHITNEY

Journal: Neurotoxicology

Article Title: Insights into cytotoxicity and redox modulation by the herbicide linuron and its metabolite, 3,4-dichloroaniline

doi: 10.1016/j.neuro.2025.103324

Figure Lengend Snippet: Linuron and DCA toxicity in N27 cells. Cells were seeded and treated with media containing serum (A, B), while cells were seeded in media with serum but treated without serum (C). Cell viability was measured upon treatments with the different compounds for 24 h (A-C). Cell viability was assessed using the calcein AM assay. Data points signify mean percentage survival relative to untreated cells ± SEM, n = 15–18 from 3 independent experiments (A–C). We used non-linear regression analysis with a variable slope model to fit a logistic curve to the dose-response data, thereby determining the IC50 with a 95 % confidence interval (CI).

Article Snippet: The N27 rat dopaminergic neural cell line was derived from embryonic day 12 (E12) rat mesencephalic tissue, provided by Dr. Wei Zheng and Dr. Jean-Christophe (Chris) Rochet from Purdue University, USA.

Techniques: Calcein AM Assay

Journal: Neurotoxicology

Article Title: Insights into cytotoxicity and redox modulation by the herbicide linuron and its metabolite, 3,4-dichloroaniline

doi: 10.1016/j.neuro.2025.103324

Figure Lengend Snippet: Linuron metabolite DCA confers resistance to ferroptosis caused by different inducers. DCA protects against erastin and RSL3-induced toxicity in N27 and HT-22 cells (A-D). Cell viability and C11-BODIPY assay were measured upon treatments with the different compounds for 24 h (A-E). Cell viability was measured by calcein AM assay (A-D). Lipid peroxidation is expressed as the ratio of green to red BODIPY 581/591 C11 fluorescence (E,F). Data points signify mean fold change of intensity/percentage survival relative to untreated cells ± SEM, n = 9–15 from 3 independent experiments. Liproxstatin-1 (Lip-1) was used as a standard ferroptosis inhibitor. To calculate statistical significance, we used a two-way ANOVA for multiple comparisons (Dunnett’s multiple comparisons test). * ** * p < 0.0001, * p = 0.0120 or ns/not significant (vs control).

Article Snippet: The N27 rat dopaminergic neural cell line was derived from embryonic day 12 (E12) rat mesencephalic tissue, provided by Dr. Wei Zheng and Dr. Jean-Christophe (Chris) Rochet from Purdue University, USA.

Techniques: Calcein AM Assay, Fluorescence, Control